"impeller speed witgh mammalian cells"

Design and development of a new ambr250® bioreactor …

2021-4-13 · impeller (W), q is the fluid density (kg/m3), N is the impeller speed (rps) and D is the impeller diameter (m). The Power number is dependent on the Reynolds number, Re, of the system where for a stirred vessel it is defined as: Re ¼ qND2 l; ð4Þ where l is the dynamic viscosity (Pa s). For bioreac-tors in which animal cells whether in free ...

Turbulent shear stress—Effect on mammalian cell culture ...

1995-8-1 · At an agitation speed of 5 r/s, the cells suffered damage even in the presence of FBS. As the impeller speed was increased from 2.5 to 5 r/s, there was an approximately twofold increase in turbulent stress. Even in the presence of serum the maximum turbulent shear stress at 5 r/s is about two times the maximum turbulent stress at 2.5 r/s.

A novel scale-up strategy for cultivation of BHK-21 cells ...

2021-8-13 · The scale-up of animal cell cultivation is important but remains complex and challenging. In the present study, we propose a novel scale-up strategy for baby hamster Syrian kidney-21 (BHK-21) cell cultivation based on similar hydrodynamic environments. The hydrodynamic characteristics of the different scale bioreactors were determined by computational fluid dynamics (CFD) and further ...

Since mammalian cells are sensitive to shear, scale-up of

2021-7-30 · Shear speed of an impeller along with the diameter of the impeller blade have profound impact on the shear stress generated in the bioreactor system. Mammalian cells are very shear sensitive. So, for scale up of a small scale bioreactor where mammalian cells are used as feed, the major parameters to be considered would be the shear speed and ...

Flow, suspension and mixing dynamics in DASGIP …

2020-10-17 · bioreactor equipped with a pitched blade impeller and then performed an hBMSC proliferation culture at the just suspended speed identified. In this work cells were successfully cultured at N js, for cell culture den-sities of 1–5 × 105 cells/ml, however it was indicated that the just suspended speed may not provide sufficient oxygen mass ...

Impeller Selection for Animal Cell Culture

2010-4-15 · Impellers in animal (mammalian and insect) cell culture bioreactors essentially have to meet the same tasks as impellers in other bioreactors. However, there are special features of both free suspension and microcarrier cell culture that lead to particular agitation conditions compared to the others. Even at, the oxygen transfer requirements ...

Fluid Dynamics of a Single-Use, Stirred-Tank Bioreactor ...

2015-9-23 · Impeller tip speed also is one critical factor. Operators often apply a notional tip-speed limit of 1.5 m/s as a general guide for stirred-tank bioreactors. It is generally thought that cell damage may begin above that value (5), even

Pitched-Blade vs. Spin Filter vs. Packed-bed Basket: CHO ...

2016-4-12 · impeller types were compared using CHO cell culture: The pitched-blade impeller, the spin filter with marine-blade impeller and the packed-bed basket impeller. All experiments were performed using a New Brunswick™ CelliGen® 310 benchtop bioreactor. The …

Cell Culture Scale-Up in BioBLU c Rigid-Wall, Single

2020-9-3 · > A wide scalable tip speed zone has been identified which covers most mammalian cell culture needs. > All three vessel sizes offer a broad overlapping range of k L a values for excellent scalability. > P/V-based scale-up can be performed by maintaining constant P/V values among different vessels from 10.0 to 18.2 W/m3.

Flow Modelling in Medium-Scale Stirred-Tank …

2014-12-18 · The effect of impeller speed on the flow in an Eppendorf New Brunswick Scientific (NBS) 5 L stirred-tank reactor (STR) filled ... as bioreactors for fermentation of mammalian cell lines and other products, and are now being considered for human stem cell applications. Human stem cells are known to be sensitive to their

(PDF) Culture of insect cells in a helical ribbon impeller ...

Culture of insect cells in a helical ribbon impeller bioreactor. Biotechnology and Bioengineering, 1991. Claude Chavarie. Jacques Archambault. Antoine Caron. Jean Luc Archambault. Amine Kamen. Roseanne Tom. Bernard Massie. Claude Chavarie.

Engineering characterization of the single-use Xcellerex ...

2020-11-12 · Engineering characterization of the single-use Xcellerex XDR-2000 stirred-tank bioreactor system Intellectual Property Notice: The Biopharma business o f GE Healthcare was acquired by Danaher on 31 March 2020 and now operates under the Cytiva™ brand.

Optimization of agitation speed in spinner flask for ...

2019-5-15 · expansion of pluripotent cells. A successful microcar-rier expansion is dependent on several factors includ-ing the type of carrier used, the cell line being expanded, the spinner flask set up, the position of the impeller and the spin rate (revolutions per minute— RPM) used. It is …

Immobilization of Mammalian Cells

2021-8-8 · Immobilization of Mammalian Cells. Biocatalysts (e.g. cells) are regarded as immobilized when they are restricted in their motility while their metabolic or catalytic activity is maintained (Lundgren and Blu¨ml 1998). For mammalian cells different techniques for immobilizing mammalian cells have been proposed, including entrapping cells on a ...

Which Impeller Is Right for Your Cell Line?

2009-1-1 · When growing microbes or animal cells in a stirred-tank reactor, it is critical to choose the impeller type that is best suited to your process. Select the wrong impeller, …

Bioreactor for the Cultivation of Mammalian Cells

The bioreactor for the cultivation of mammalian cells according to the invention has preferably a volume of at least 4000 l and at least one top impeller and at least one bottom impeller, wherein the top impeller is a hydrofoil impeller. In a preferred embodiment of the invention the top impeller is a hydrofoil impeller.

Optimizing CHO Cell Culture Conditions

2009-10-1 · Initially the effect of impeller speed (80, 110, 140, and 170 rpm) on the cells was tested (Figures 2A and 2B). Increased impeller speeds improve the oxygen transfer rate in the growth medium, but ...

Stirred Bioreactors: Current State and Developments, With ...

2017-1-1 · As ultra-high cell densities (>1 × 10 8 cells/mL) and very high product titers (>10 g/L) have been generated without laborious process optimization,, concentrated fed-batch mode is regarded as the most simple and efficient perfusion strategy to increase the space time yield rapidly in stirred bioreactors for mammalian cell-based ...

Animal Cell Culture in Stirred Bioreactors: Observations ...

2005-12-2 · The general course of a cell culture run is shown in Fig. 1. The culture peaked around 45- 50 h, a cell concentration of almost 2 x 10" cells mP3 was attained (at a constant impeller speed of 2 s-l) and a fairly high and stable cell viability ( > 80%) was maintained until the decline

Everything You Ever Wanted to Know About Cell Culture ...

2019-11-21 · 1. Oxygen transfer: Since mammalian cell culture processes are aerobic, the oxygen transfer into the cells is a very critical factor. To ensure adequate oxygen availability, the agitation speed, gas flow rate and gas composition need to be controlled.

Optimization of agitation speed in spinner flask for ...

2015-2-6 · Optimization of agitation speed in spinner flask ... large quantitiesof cells generated invitro. Mammalian cells, including pluripotent stem cells, have been expanded using 3D culture, however current limita- ... impeller and the spin rate (revolutions per minute— ...

Impeller Selection for Animal Cell Culture | Request PDF

Cytodex 3 microcarriers were not damaged when held at the minimum speed, N(JS), for their suspension, using either impeller, nor was there any observable damage to the cells.

High-density mammalian cell cultures in stirred-tank ...

2016-8-10 · Interactions between CO 2, lactate, and pH were leveraged to maintain culture pH.. No external pH control required in both fed-batch and perfusion cultures in stirred-tank bioreactors. • Cell specific perfusion rate at as low as 15 ± 2 pL/cell/day was achieved in perfusion cultures.. High bioreactor productivity at 1.9 ± 0.1 g/L/day was achieved in perfusion cultures.

Introduction

2008-10-9 · Equation 5 illustrates how this impeller constant influences the amount of power delivered to the cells. Power = (impeller constant) * (N I) 3 * (D I) 5 Equation 5. In this equation N I is the impeller stirring speed in RPS, and D I is the impeller diameter in centimeters. The power delivered to the cell culture affects how well oxygen is distributed throughout the media (Doran, 151).

Fluid Dynamics of a Single-Use, Stirred-Tank Bioreactor for M…

Optimizing CHO Cell Culture Conditions

N90-13955

2020-8-6 · and operation of a bioreactor is illustrated by the JSC mammalian cell culture system. The design of a bioreactor is strongly dependent upon its intended use as a production unit for cell mass and/or biologicals or as a research reactor for the study of cell growth and function. Therefore a …

BIOREACTOR MODELLING & CONTROL

2014-3-28 · involve the culture of living organisms such as bacteria, fungi, algae or mammalian cells in order to achieve a diverse range of outcomes including wastewater treatment, beer brewing, manufacture of vaccines or pharmaceutical agents, or simply the reproduction of cells, known as biomass ... air or oxygen underneath a speed-controlled impeller ...

Criteria for bioreactor comparison and operation ...

2011-11-22 · Criteria for process transfer were analyzed during the cultivation the human production cell line AGE1.HN. Growth was compared within ranges for power input, mixing time, impeller tip speed and Reynolds number. Maximum specific growth rates were observed for AGE1.HN cells at a common mixing time range of 8 - 13 seconds for all cultivation systems.

How do I calculate impeller speed of a bioreactor from ...

Hi, as far as I know, the power number is a dimensionless number relating the resistance force to the inertia force, and it is independent of the impeller speed under turbulent regime (Re>4000), I ...

Stirred Tank Reactors

2021-6-26 · In the literature, a number of scale-up criteria valid for agitation rate and tolerable shear forces are discussed such as impeller tip speed (61), integrated shear factor (62), or Kolmogorov''s theory of isotropic turbulence (63-65). For hybridoma cells …

Evaluation of criteria for bioreactor comparison and ...

2012-7-9 · Development of bioprocesses with mammalian cell culture deals with different bioreactor types and scales. The bioreactors might be intended for generation of cell inoculum and production, research, process development, validation, or transfer purposes. ... In this work, power input, mixing time, impeller tip speed, and Reynolds number have been ...

High shear resistance of insect cells: the basis for ...

2021-5-3 · The theory that mammalian or insect cells are highly sensitive to shear is widespread and persistent, although there is no sound scientific base supporting this hypothesis. ... the impeller speed ...

Stirred Tank Reactors

2021-6-26 · In the literature, a number of scale-up criteria valid for agitation rate and tolerable shear forces are discussed such as impeller tip speed (61), integrated shear …